Cordycepin benefit and review of studies
Cordyceps militaris, a traditional medicinal mushroom, produces a component compound, cordycepin (3'-deoxyadenosine). Cordycepin has been known to have many pharmacological activities including immunological stimulating, anti-cancer, and anti-infection activities.
Pharmacokinetics of Adenosine and Cordycepin, a Bioactive Constituent of
Cordyceps sinensis in Rat.
J Agric Food Chem. 2010. Institute of Traditional Medicine, School of
Medicine, National Yang-Ming University, Taipei, Taiwan.
Cordycepin is a bioactive constituent of Cordyceps sinensis that has been shown
to regulate homeostatic function. As an adenosine analogue, it is possible
cordycepin goes through a similar metabolic pathway to that of adenosine. To
investigate this hypothesis, a sensitive liquid chromatography with
photodiode-array detector (HPLC-PDA) coupled to a microdialysis sampling system
was developed to monitor cordycepin and adenosine in rat blood and liver. Other
endogenous nucleosides were simultaneously measured to further understand the
downstream metabolic pathway. The experiments were divided into six parallel
groups for drug administration: (1) normal saline vehicle, (2) adenosine, (3)
cordycepin, (4) normal saline + erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA; a
potent adenosine deaminase inhibitor), (5) adenosine + EHNA, and (6) cordycepin
+ EHNA. The pharmacokinetic results suggest that the levels of both adenosine
and cordycepin decreased rapidly in blood around 30 min after drug
administration. When adenosine was given, the concentrations of adenosine
metabolites, hypoxanthinosine and hypoxanthine, increased in rat blood. This
phenomenon was inhibited by EHNA pretreatment. An unidentified peak was observed
in the blood and liver samples after cordycepin administration. The decline of
this unidentified peak paralleled the decreased of the concentration of
cordycepin, and it was not observed in the presence of the adenosine deaminase
inhibitor. It is concluded that adenosine and cordycepin had short elimination
half-lives and high rates of clearance and their biotransformation was
suppressed by EHNA.
Cordycepin Suppresses Expression of Diabetes Regulating Genes by Inhibition
of Lipopolysaccharide-induced Inflammation in Macrophages.
Immune Netw. 2009. College of Pharmacy, Sahmyook University,
Seoul, Korea.
It has been recently noticed that type 2 diabetes (T2D), one of the most common
metabolic diseases, causes a chronic low-grade inflammation and activation of
the innate immune system that are closely involved in the pathogenesis of T2D.
In the present study, we tested the role of cordycepin on the anti-diabetic
effect and anti-inflammatory cascades in LPS-stimulated RAW 264.7 cells. We
confirmed the levels of diabetes regulating genes mRNA and protein of cytokines
through RT-PCR and western blot analysis and followed by FACS analysis for the
surface molecules. Cordycepin inhibited the production of NO and
pro-inflammatory cytokines such as IL-1beta, IL-6, and TNF-alpha in
LPS-activated macrophages via suppressing protein expression of pro-inflammatory
mediators. T2D regulating genes such as 11beta-HSD1 and PPARgamma were decreased
as well as expression of co-stimulatory molecules such as ICAM-1 and B7-1/-2
were also decreased with the increment of its concentration. In accordance with
suppressed pro-inflammatory cytokine production lead to inhibition of diabetic
regulating genes in activated macrophages. Cordycepin suppressed NF-kappaB
activation in LPS-activated macrophages. Based on these observations, cordycepin
suppressed T2D regulating genes through the inactivation of NF-kappaB dependent
inflammatory responses and suggesting that cordycepin will provide potential use
as an immunomodulatory agent for treating immunological diseases.